Simple Western™ analysis of lysates (0.1 mg/mL) from 3T3 cells using LC3A/B (D3U4C) XP® Rabbit mAb #12741. The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 2 - 40 kDa separation module.
| Cat. # | Size | Qty. | Price |
|---|---|---|---|
| 4445T | 1 Kit (7 x 20 microliters) |
|
| Product Includes | Quantity | Applications | Reactivity | MW(kDa) | Isotype |
|---|---|---|---|---|---|
| Beclin-1 (D40C5) Rabbit mAb 3495 | 20 µl |
|
H M R Mk | 60 | Rabbit IgG |
| LC3A/B (D3U4C) XP® Rabbit mAb 12741 | 20 µl |
|
H M R | 14, 16 | Rabbit IgG |
| Atg5 (D5F5U) Rabbit mAb 12994 | 20 µl |
|
H M R | 55 | Rabbit IgG |
| Atg12 (D88H11) Rabbit mAb 4180 | 20 µl |
|
H M R Mk | 16, 55 | Rabbit IgG |
| Atg16L1 (D6D5) Rabbit mAb 8089 | 20 µl |
|
H M R | 66, 68 | Rabbit IgG |
| Atg7 (D12B11) Rabbit mAb 8558 | 20 µl |
|
H M R | 78 | Rabbit IgG |
| Atg3 Antibody 3415 | 20 µl |
|
H M R Mk | 40 | Rabbit |
| Anti-rabbit IgG, HRP-linked Antibody 7074 | 100 µl |
|
Goat |
Product Information
Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Leu44 of human LC3B, Thr72 of human Beclin-1, Leu265 of human Atg5, Ser36 of human Atg12, or near the amino termini of human Atg7 and Atg16L1. Polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues near the amino-termini of human Atg3. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.
Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10 (7,8).
Explore pathways related to this product.
STRING - Known and Predicted Protein-Protein Interactions.
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