| Cat. # | Size | Qty. | Price |
|---|---|---|---|
| 52239T | 1 Kit (9 x 20 microliters) |
|
| Product Includes | Quantity | Applications | Reactivity | MW(kDa) | Isotype |
|---|---|---|---|---|---|
| Phospho-STING (Ser366) (D7C3S) Rabbit mAb 19781 | 20 µl |
|
H | 40 | Rabbit IgG |
| STING (D2P2F) Rabbit mAb 13647 | 20 µl |
|
H M Hm | 33, 35 | Rabbit IgG |
| Phospho-IRF-3 (Ser396) (D6O1M) Rabbit mAb 29047 | 20 µl |
|
H M R | 45-55 | Rabbit IgG |
| IRF-3 (D6I4C) XP® Rabbit mAb 11904 | 20 µl |
|
H Mk | 50-55 | Rabbit IgG |
| Phospho-IRAK4 (Thr345/Ser346) (D6D7) Rabbit mAb 11927 | 20 µl |
|
H | 55 | Rabbit IgG |
| IRAK4 Antibody 4363 | 20 µl |
|
H M R Mk | 55 | Rabbit |
| Phospho-IRF-7 (Ser477) (D7E1W) Rabbit mAb 12390 | 20 µl |
|
H | 65 | Rabbit IgG |
| IRF-7 (D2A1J) Rabbit mAb 13014 | 20 µl |
|
H | 65 | Rabbit IgG |
| Cleaved-IL-1β (Asp116) (D3A3Z) Rabbit mAb 83186 | 20 µl |
|
H | 17 | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody 7074 | 100 µl |
|
Goat |
Product Information
Monoclonal and polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Pro226 of STING, Lys41 of IRAK4, Pro115 of IRF-7, Asp116 of IL-1β, or recombinant human IRF-3 protein. Phospho-specific antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Ser366 of STING, Ser396 of IRF-3, Thr345/Ser346 of IRAK4, or Ser477 of IRF-7. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.
The innate immune system responds rapidly to pathogens by detecting conserved pathogen-associated molecular patterns (PAMPs) and damage/danger-associated molecular patterns (DAMPs) through pattern recognition receptors (PRRs). There are several families of PRRs. Toll-like receptors (TLRs) are transmembrane PRRs and signal through recruitment of adaptor proteins, including MyD88, which leads to recruitment and phosphorylation of IRAK1 and IRAK4, followed by activation of NF-κB and MAP kinases (1-3). Some TLRs also activate IRFs, which upregulate the type I interferon response. Activation of TLR3 and TLR4 results in phosphorylation and activation of IRF-3, while TLR7, TLR8, and TLR9 lead to activation of IRF-7 (2, 3). STING is a multi-pass ER transmembrane protein that is activated in response to intracellular DNA downstream of DNA-sensing cytoplasmic PRRs, such as DDX41, or by binding the second messenger cyclic-GMP-AMP (cGAMP) produced by cGAS (4-6). Following activation, STING translocates with TBK1 to perinuclear endosomes, leading to phosphorylation and activation of IRF-3 and NF-κB (7, 8). Following activation and translocation, STING gets phosphorylated by ULK1, resulting in STING inactivation and degradation (9). Inflammasomes are cytoplasmic multimeric protein complexes that assemble in response to PAMPs or DAMPs detected by AIM2 or members of the nod-like receptor (NLR) family, such as NLRP3 (10). Inflammasomes activate Caspase-1, which cleaves the IL-1β and IL-18 precursor proteins into the mature forms (10).
Explore pathways related to this product.
STRING - Known and Predicted Protein-Protein Interactions.
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