Cat. # | Size | Qty. | Price |
---|---|---|---|
96628T | 1 Kit (6 x 20 microliters) |
|
Product Includes | Quantity | Applications | Reactivity | MW(kDa) | Isotype |
---|---|---|---|---|---|
Tau (D1M9X) XP® Rabbit mAb 46687 | 20 µl |
|
H M R | 50-80 | Rabbit IgG |
Phospho-Tau (Ser202) (D4H7E) Rabbit mAb 39357 | 20 µl |
|
H M R | 50-80 | Rabbit IgG |
Phospho-Tau (Ser396) (PHF13) Mouse mAb 9632 | 20 µl |
|
M R | 50-80 | Mouse IgG2b |
Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb 20194 | 20 µl |
|
H M R | 50-80 | Rabbit IgG |
Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb 35834 | 20 µl |
|
H M R | 50-80 | Rabbit IgG |
Phospho-Tau (Ser416) (D7U2P) Rabbit mAb 15013 | 20 µl |
|
H M R | 50-80 | Rabbit IgG |
Anti-rabbit IgG, HRP-linked Antibody 7074 | 100 µl |
|
Goat | ||
Anti-mouse IgG, HRP-linked Antibody 7076 | 100 µl |
|
Horse |
Product Information
Monoclonal antibodies are produced by immunizing rabbits with synthetic peptides corresponding to residues surrounding Asp430 of human Tau. Phosphorylation-specific monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to Ser400/Thr403/Ser404, Thr181, Ser202, Ser416 Tau, and Ser396 with human purified Tau (Hoffmann et al., 1997 -PMID 9201960).
Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by Erk, glycogen synthase kinase-3 (GSK-3), and CDK5 (1,2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease (AD); these tangles are bundles of paired helical filaments (PHFs) composed of hyperphosphorylated tau. In particular, phosphorylation at Ser396 by GSK-3 or CDK5 destabilizes microtubules. Furthermore, research studies have shown that inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3).
Investigators have shown that Tau phosphorylation at Ser404 destabilizes microtubules and that Tau is hyperphosphorylated at Ser404 in Alzheimer's disease (2,4-6). Research studies indicate that calcium-/calmodulin-dependent protease kinase II (CAM-kkinase II) is responsible for the phosphorylation of Tau at Ser416. Phosphorylated Tau protein is localized with neuronal soma or hippocampal neurons and immortalized GnRH neurons (7). Investigators have shown that Tau is phosphorylated during development and hyperphosphorylated at Ser202 in Alzheimer's disease (8).
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.